Tranquill was the first Pokémon Trip used in his second battle with Ash in the Battle Club. It easily defeated Ash's Oshawott with its Super Luck-boosted Aerial Ace. Ash sent Tepig, who attacked with Ember, but missed due to Tranquill's Double Team, who boosted itself by using Work Out. Like Oshawott, Tepig was also defeated by Tranquill's Aerial Ace. Ash sent Pikachu in the battle, who attempted to use Thunderbolt, but missed. Instead, Tranquill boosted itself by using Work Out and dealt damage to Pikachu with Aerial Ace. Tranquill repeated its attack, but Pikachu managed to grab its leg and toss it away and defeat it with Volt Tackle attack.
Tranquill saved Ash's Pidove by using Air Cutter to block a Venipede's Sludge Bomb in Castelia City. It then teamed up with Ash's newly evolved Tranquill, using Aerial Ace to send the other Venipede back to their colony.