Pikachutwo differs from Ash's Pikachu with the slur in his voice and the black spiked patterns on its ears. This may have been caused by Ash disrupting the cloning process when Pikachu was being cloned.
Mewtwo Strikes Back
After he was created, Pikachutwo attacked Ash's Pikachu, who didn't fight back. However, the battle came to a stop when Ash ran between Mewtwo's and Mew's attacks, which turned him to stone. Like all the other Pokémon, Pikachu felt utterly miserable about it.
However, the Pokémon's tears healed Ash. Mewtwo used his psychic abilities to lift Pikachutwo and the other clone Pokémon in the air, and they went away to find a safe home.
The clones had found a lovely place to live, at Johto's largest mountain at Mount Quena. Pikachutwo is seen with Meowthtwo, talking to Mewtwo. He was also seen saying no to Mewtwo right before Mewtwo's torture.
Later, after Team Rocket attempts to steal Ash's Pikachu, Pikachutwo comes to the place they crashed in. He uses Thunderbolt to stop Ash's Pikachu's Thunderbolt on Team Rocket. Then Pikachutwo goes on about how angry he is and tells Ash's Pikachu to leave. Meowth translates it all, one of the things being "Get out, or you'll regret it!" However, when Pikachutwo is about to attack, Mewtwo stops him by using psychic powers.
- This is the only Pokémon in the anime to be owned by another Pokémon.