Steelix is Jasmine's powerhouse Pokémon and she used it to stop Team Rocket. Using Crunch, Steelix stopped Team Rocket's machine and blasted them away with Iron Tail. In her Gym battle with Ash Ketchum, Steelix used Iron Tail but missed Pikachu, who used Quick Attack. Since it was worn out from the last battle, Pikachu was easily defeated with Steelix's powerful Iron Tail. Ash's Cyndaquil was sent out against Steelix, who used Sandstorm to block Cyndaquil's Flamethrower. Steelix Dig within the Sandstorm and used Iron Tail, which Cyndaquil evaded. Cyndaquil repeated its attack, but Steelix also repeated its moves and nearly hit Cyndaquil. Steelix repeated its Iron Tail, but Cyndaquil used Flamethrower on the wall to bounce off, while Steelix used Dig. Cyndaquil took the opportunity and used Smokescreen, causing Steelix to miss, as Cyndaquil ran into one of Steelix's holes it dug out. Steelix's Sandstorm blew away the smoke and made Jasmine and Steelix see Cyndaquil was gone. Cyndaquil emerged out and used Flamethrower, burning Steelix before going back into the hole. By using Iron Tail on the ground, Steelix collapsed the holes and made Cyndaquil emerge out. Eventually, it smashed Cyndaquil, who tried to use Flamethrower to bounce away, with a devastating Iron Tail, almost defeating it. Regardless, Cyndaquil stood up and used Smokescreen to hide away from Steelix's Crunch. To finish the battle, Cyndaquil launched the strongest Flamethrower it could muster, roasting Steelix within its own Sandstorm and defeating it.
When Team Rocket stole Ash's badges, Ash was searching for the lost badges. He also remembered his battles with the Johto Gym Leaders, including Jasmine and her Steelix, as Ash and his Pokémon won those badges through hard effort.
Jasmine challenged Flint to a battle and sent her Steelix out. Ash recalled how his Pikachu battled Steelix, whose Iron Tail was a powerful move. Steelix used Iron Tail and repeated its attack, but Flint's Infernape avoided the attack. Instead, Steelix started a Sandstorm, but Infernape steered clear from the sandstorm. Steelix attempted to use Crunch, but was immediately defeated by Flint's Infernape's Flare Blitz.
- Unshō Ishizuka, who plays the Narrator in the Japanese version, voices Steelix in both the Japanese and English-language anime.