Gardenia's Turtwig first appeared while Ash and his friends were heading towards Eterna City. When Gardenia challenged Ash, she used Turtwig, who displayed incredible speed, being about twice as fast as Ash's Turtwig, who was also quite agile for a Turtwig. Turtwig defeated Ash's Turtwig and Staravia without taking any damage, even managing to avoid Staravia's Aerial Ace.
Turtwig was later used to help protect the Adamant Orb.
During Ash's second Gym battle with Gardenia, Turtwig used his incredible speed to defeat Staravia for the second time. Turtwig then battled Ash's Turtwig again but this time, was defeated.
Later, when Gardenia and James challenged Ash and Dawn to a double battle, Turtwig fought alongside Gardenia's Cacnea. However, Gardenia lost focus during the battle, resulting in Turtwig being defeated by Dawn's Pachirisu.